PMID: 8485905, 3040333
Both zymosan (cell wall extract from the yeast Saccharomyces cereviseae) and Escherichia coli bind to resting CR3 and
promote the phagocytically active state of CR3.
beta-glucan component of zymosan, was responsible for CR3-dependent responses to iC3b-opsonized zymosan.
CR3-dependent responses to fl-glucan were blocked by the CR3 alpha chain-specific MoAb OKMI.
PMID: 8485905
Both zymosan (cell wall extract from the yeast Saccharomyces cereviseae) and Escherichia coli bind to resting CR3 and
promote the phagocytically active state of CR3.
PMID: 8485905, 833545, 573303, 7430626, 7252421, 7049467
Fixed iC3b on sheep erythrocytes (EC3bi) binds avidly to neutrophil, monocyte, and NK cell CR3, but does not trigger any cytotoxic reaction.
PMID: 8485905, 3537192
Lipopolysaccharide (LPS) on E. coli appears to bind to CR3 at a distinct site from beta-glucan and also stimulates CR3-dependent phagocytosis.
PMID: 8485905, 1682263
certain fimbriated strains of E. coli stimulate CR3- dependent phagocytosis by way of a mannose-specific lectin on the bacteria that binds to a carbohydrate on CR3.
indirect
PMID: 8485905, 1383326, 3283242, 1976698
Neutrophil homotypic aggregation requires PKC for exposure of a CR3 binding site for a counter-receptor on other neutrophils recently shown to be L-selectin.
PMID: 8485905, 1976698, 1673986
CR3 is capable of mediating phagocytosis following protein kinase C (PKC)-dependent activation.
Blockade of both PMA- induced phagocytosis and homotypic aggregation by the PKC inhibitor staurosporine suggests a similar activation pathway for these two CR3-dependent functions.
Neutrophil aggregation and phagocytosis of EC3bi induced by PMA were blocked by staurosporine suggested that a PKC-associated phosphorylation event was required for CR3 activation.
beta-glucan derived from zymosan binds to CR3 and activates the receptor via a phosphorylation event involving both PKC and a tyrosine kinase.
PMID: 8485905, 2579146
soluble glucans from barley or laminarin bound weakly to CR3.
indirect
PMID: 8485905
beta-glucan derived from zymosan binds to CR3 and activates the receptor via a phosphorylation event involving both PKC and a tyrosine kinase.
glucan-induced phagocytosis of EC3bi is not only prevented by staurosporine,but it is also blocked by genistein or herbimycin A, two tyrosine kinase inhibitors.