/home/ikeda/eclipse/workspace/macrophage/csml/ok/csml/1373512.csml - 2009.07.01.10:42

Original Literature	Model OverView
Publication Title Function of lipopolysaccharide (LPS)-binding protein (LBP) and CD14, thereceptor for LPS/LBP complexes: a short review. Authors Schumann RR. Affiliation Department of Internal Medicine I, Albert-Ludwigs-University of Freiburg,Germany. Abstract With the recent discovery and cloning of the lipopolysaccharide-binding protein(LBP), the "adapter-molecule" for LPS-binding to the cell surface receptor CD14was found. The ligand-receptor pair LPS/LBP-CD14 seems to be one importantelement in LPS-mediated activation of monocytic cells and possibly granulocytesand B cells. Here, some of the known functions of the proteins involved, LBP andCD14, are reviewed in the context of other endotoxin recognition studies, andthe outlook for ongoing and future investigations is described. PMID 1373512

Model Detail

57.75, 122.67311985969587

812.4052907834925, 1179.6124822176303

cso30:c:EntityBiologicalCompartment

cso30:i:CC_PlasmaMembrane

csml-variable:Double

false

infinite

unit

Go back

65.57746841077729, 140.70295043735976

787.4695067824475, 1107.7481717682972

e10

cso30:c:EntityBiologicalCompartment

cso30:i:CC_Cytosol

csml-variable:Double

m10

false

infinite

unit

Go back

1042.5, 294.0

42.36977170471589, 51.88135310781536

LPS:LBP

e11

cso30:c:Complex

cso30:i:CC_Extracellular

p2 p3 p4

csml-variable:Double

m11

false

infinite

unit

Go back

204.0708721348408, 757.256350634969

453.5808752173955, 411.0847702735097

e12

cso30:c:EntityBiologicalCompartment

cso30:i:CC_NuclearInnerMembrane

csml-variable:Double

m12

false

infinite

unit

Go back

198.0, 751.6191122240452

465.72261948707705, 422.35924709535703

e13

cso30:c:EntityBiologicalCompartment

cso30:i:CC_NuclearEnvelope

csml-variable:Double

m13

false

infinite

unit

Go back

204.93813958267532, 758.1236180828032

451.8463403217266, 409.3502353778407

e14

cso30:c:EntityBiologicalCompartment

cso30:i:CC_NuclearLumen

csml-variable:Double

m14

false

infinite

unit

Go back

248.30151197439562, 761.6284123664391

272.32197862000385, 409.3502353778407

e15

cso30:c:EntityBiologicalCompartment

cso30:i:CC_NuclearPore

csml-variable:Double

m15

false

infinite

unit

Go back

198.0, 751.6191122240452

465.72261948707705, 422.35924709535703

e16

cso30:c:EntityBiologicalCompartment

cso30:i:CC_Nucleus

csml-variable:Double

m16

false

infinite

unit

Go back

204.93813958267532, 758.1236180828032

451.8463403217266, 409.3502353778407

e17

cso30:c:EntityBiologicalCompartment

cso30:i:CC_Nucleoplasm

csml-variable:Double

m17

false

infinite

unit

Go back

198.0, 751.6191122240452

465.72261948707705, 422.35924709535703

e18

cso30:c:EntityBiologicalCompartment

cso30:i:CC_NuclearEnvelopeLumen

csml-variable:Double

m18

false

infinite

unit

Go back

461.64930414165997, 1046.4900444877449

82.39040754426873, 74.58500051375918

e19

cso30:c:EntityBiologicalCompartment

cso30:i:CC_Nucleolus

csml-variable:Double

m19

false

infinite

unit

Go back

57.75, 122.67311985969587

812.4052907834925, 1179.6124822176303

cso30:c:EntityBiologicalCompartment

cso30:i:CC_PlasmaMembrane_ExternalSideOfPlasmaMembrane_

csml-variable:Double

false

infinite

unit

Go back

198.0, 751.6191122240452

465.72261948707705, 422.35924709535703

e20

cso30:c:EntityBiologicalCompartment

cso30:i:CC_NuclearOuterMembrane

csml-variable:Double

m20

false

infinite

unit

Go back

537.9688395510874, 925.0726017909269

29.09579035072251, 26.339347054338326

e21

cso30:c:EntityBiologicalCompartment

cso30:i:CC_NuclearBody

csml-variable:Double

m21

false

infinite

unit

Go back

307.76820097229336, 939.3120716500325

14.748751051648703, 7.101250506349382

e22

cso30:c:EntityBiologicalCompartment

cso30:i:CC_Chromatin

csml-variable:Double

m22

false

infinite

unit

Go back

328.4533078689168, 977.234767627175

7.880040722523023, 7.880040722523032

e23

cso30:c:EntityBiologicalCompartment

cso30:i:CC_NuclearCentromere

csml-variable:Double

m23

false

infinite

unit

Go back

307.2756984271356, 918.134462208252

50.48151087866315, 90.86671958159374

e24

cso30:c:EntityBiologicalCompartment

cso30:i:CC_NuclearChromosome

csml-variable:Double

m24

false

infinite

unit

Go back

503.0, 829.0

57.19723183391003, 30.505190311418687

TNF

e25

cso30:c:mRNA

cso30:i:CC_Nucleoplasm

p3 p5

csml-variable:Double

m25

false

infinite

unit

Go back

736.0, 669.78

48.96000000000001, 36.72000000000001

TNF

e26

cso30:c:Protein

cso30:i:CC_Cytosol

p3 p5

csml-variable:Double

m26

false

infinite

unit

Go back

816.5, 358.0

39.0, 60.0

CD14

e27

cso30:c:Protein

cso30:i:CC_PlasmaMembrane_IntegralToPlasmaMembrane_

csml-variable:Double

m27

false

infinite

unit

Go back

810.5, 506.0

43.0, 60.0

LPS:LBP:CD14

e28

cso30:c:Complex

cso30:i:CC_Cytosol

csml-variable:Double

m28

false

infinite

unit

Go back

586.0, 540.1836385590663

52.4049104447575, 32.316361440933775

3C10

e29

cso30:c:Protein

cso30:i:CC_Cytosol

p3 p5

csml-variable:Double

m29

false

infinite

unit

Go back

73.99810581566987, 138.92122567536578

779.9090791521529, 1147.1162705862912

cso30:c:EntityBiologicalCompartment

cso30:i:CC_PlasmaMembrane_IntegralToPlasmaMembrane_

csml-variable:Double

false

infinite

unit

Go back

686.0, 463.26000000000005

44.31999999999999, 33.24000000000001

60b

e30

cso30:c:Protein

cso30:i:CC_Cytosol

p3 p5

csml-variable:Double

m30

false

infinite

unit

Go back

1342.5, 75.56208492654457

34.80338096196418, 56.43791507345543

albumin

e31

cso30:c:Protein

cso30:i:CC_Extracellular

csml-variable:Double

m31

false

infinite

unit

Go back

1339.0, 172.53591160220995

41.81767955801105, 46.46408839779006

LPS:albumin

e32

cso30:c:Complex

cso30:i:CC_Extracellular

csml-variable:Double

m32

false

infinite

unit

Go back

1008.0, 80.5

39.99999999999999, 30.0

ComplementC1

e33

cso30:c:Protein

cso30:i:CC_Extracellular

csml-variable:Double

m33

false

infinite

unit

Go back

1344.5, 260.3779370104982

38.89135144142643, 47.62206298950175

LPS:complementC1

e34

cso30:c:Complex

cso30:i:CC_Extracellular

csml-variable:Double

m34

false

infinite

unit

Go back

949.0, 137.11862917398946

45.20210896309315, 29.381370826010546

complement C3

e35

cso30:c:Protein

cso30:i:CC_Extracellular

p10

csml-variable:Double

m35

false

infinite

unit

Go back

376.5, 118.0

39.0, 60.0

CD11:CD18

e36

cso30:c:Protein

cso30:i:CC_PlasmaMembrane_IntegralToPlasmaMembrane_

p12

csml-variable:Double

m36

false

infinite

unit

Go back

1317.0, 354.4033149171271

38.33701657458563, 42.59668508287294

LPS:complement C3

e37

cso30:c:Complex

cso30:i:CC_Extracellular

p10

csml-variable:Double

m37

false

infinite

unit

Go back

1048.5, 450.30750654055146

31.877037633326623, 51.692493459448585

lipoproteins

e38

cso30:c:Protein

cso30:i:CC_Extracellular

p11

csml-variable:Double

m38

false

infinite

unit

Go back

1323.5, 451.2382936177304

40.63872687885352, 49.76170638226962

LPS:lipoproteins

e39

cso30:c:Complex

cso30:i:CC_Extracellular

p11

csml-variable:Double

m39

false

infinite

unit

Go back

104.05710157465914, 167.35541085278783

719.7910876341746, 1090.2479002314462

cso30:c:EntityBiologicalCompartment

cso30:i:CC_PlasmaMembrane_InternalSideOfPlasmaMembrane_

csml-variable:Double

false

infinite

unit

Go back

528.5, 118.0

31.0, 60.0

LPS:CD11:CD18

e40

cso30:c:Complex

cso30:i:CC_PlasmaMembrane_IntegralToPlasmaMembrane_

p12

csml-variable:Double

m40

false

infinite

unit

Go back

745.5, 122.0

37.0, 60.0

Unknown protein

e41

cso30:c:Protein

cso30:i:CC_PlasmaMembrane_IntegralToPlasmaMembrane_

p13

csml-variable:Double

m41

false

infinite

unit

Go back

811.5, 186.0

49.0, 60.0

LPS:unknown protein

e42

cso30:c:Complex

cso30:i:CC_Extracellular

p13

csml-variable:Double

m42

false

infinite

unit

Go back

1158.0, 15.0

60.0, 34.0

LPS

cso30:c:SmallMolecule

cso30:i:CC_Extracellular

p1 p8 p9 p10 p11 p12 p13

csml-variable:Double

false

infinite

unit

Go back

926.0, 217.5

60.0, 37.0

LBP

cso30:c:Protein

cso30:i:CC_Extracellular

csml-variable:Double

false

infinite

unit

Go back

68.42382056015084, 127.41997374028314

796.7164864702795, 1128.9266819357465

cso30:c:EntityBiologicalCompartment

cso30:i:CC_Cell

csml-variable:Double

false

infinite

unit

Go back

71.2701727095244, 147.34443878589803

764.2996189616421, 1109.7630467323045

cso30:c:EntityBiologicalCompartment

cso30:i:CC_Cell_WithoutCellWall_

csml-variable:Double

false

infinite

unit

Go back

78.8604451078539, 167.26890383151297

760.8101719564544, 1095.2087824558687

cso30:c:EntityBiologicalCompartment

cso30:i:CC_Cytoplasm

csml-variable:Double

false

infinite

unit

Go back

1053.0, 225.0

21.0, 21.0

cso30:i:ME_Binding

cso30:i:CC_Extracellular

e6 e5

e11

and

mass

coefficient1:0.1
coefficient2:1.0
stoichiometry:c1 : 1
stoichiometry:c2 : 1
stoichiometry:c3 : 1

m6*m5*0.1

nodelay

true

false

PMID: 1373512 LBP, which does not have activity by itself, binds to LPS with high affinity.

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1132.0, 224.0

23.0, 23.0

p10

cso30:i:ME_Binding

cso30:i:CC_Extracellular

e35 e5

e37

and

mass

coefficient1:0.1
coefficient2:1.0
stoichiometry:c27 : 1
stoichiometry:c28 : 1
stoichiometry:c29 : 1

m35*m5*0.1

nodelay

true

false

PMID: 1373512, 2254981 Known serum proteins that bind to lipid A moiety of LPS include albumin, the complement proteins Cl and C3 and detoxifying as well as a down-modulating effect on LPS activities.

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1176.0, 463.5

24.5, 24.5

p11

cso30:i:ME_Binding

cso30:i:CC_Extracellular

e38 e5

e39

and

mass

coefficient1:0.1
coefficient2:1.0
stoichiometry:c30 : 1
stoichiometry:c31 : 1
stoichiometry:c32 : 1

m38*m5*0.1

nodelay

true

false

Go back

499.0, 19.0

25.0, 25.0

p12

cso30:i:ME_Binding

cso30:i:CC_Extracellular

e36 e5

e40

and

mass

coefficient1:0.1
coefficient2:1.0
stoichiometry:c33 : 1
stoichiometry:c34 : 1
stoichiometry:c35 : 1

m36*m5*0.1

nodelay

true

false

PMID: 1373512, 3537192 Evidence exists that, on the cell surface, the CD1l/CD18 Leu-CAM complex also binds directly to LPS.

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865.0, 77.5

24.5, 24.5

p13

cso30:i:ME_Binding

cso30:i:CC_Extracellular

e41 e5

e42

and

mass

coefficient1:0.1
coefficient2:1.0
stoichiometry:c36 : 1
stoichiometry:c37 : 1
stoichiometry:c38 : 1

m41*m5*0.1

nodelay

true

false

PMID: 1373512, 2456339, 2345180, 3170565 A 73-kDa protein that is present on many cell types, an I8-kDa protein in the membrane of 702/3 cells and two proteins of 95 and 31 kDa in the membranes of RAW 263.1 cells have also recently been identified as binding to LPS.

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357.0, 829.0

30.0, 30.0

cso30:i:ME_GeneExpression

cso30:i:CC_Nucleoplasm

e11

e25

and

mass

coefficient1:0.1
coefficient2:1.0
stoichiometry:c8 : 1
stoichiometry:c4 : 1

m11*0.1

nodelay

true

false

PMID: 1373512,2402637 LPS-induced TNF production and TNF-mRNA expression in rabbit peritoneal macrophages, for exampie, is enhanced strongly when LPS is complexed to LBP.

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746.0, 830.0

27.0, 27.0

cso30:i:ME_Translation

cso30:i:CC_Cytosol

e25 e11 e30 e29

e26

and

mass

coefficient1:0.1
coefficient2:1.0
stoichiometry:c5 : 1
stoichiometry:c7 : 1
stoichiometry:c39 : 1
stoichiometry:c19 : 1
stoichiometry:c6 : 1

m25*m11*0.1

nodelay

true

false

PMID: 1373512,2402637 LPS-induced TNF production and TNF-mRNA expression in rabbit peritoneal macrophages, for exampie, is enhanced strongly when LPS is complexed to LBP. PMID: 1373512, 1698311 In experiments that gave first evidence for CD14 being the receptor for LBP/LPS complexes, anti-CD 14 mAb 3C IO and 6Ob were able to inhibit LBP/LPS-mediated effects, including TNF production and opsonin function.

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1050.0, 374.0

27.0, 27.0

cso30:i:ME_Binding

cso30:i:CC_Extracellular

e27 e11

e28

and

mass

coefficient1:0.1
coefficient2:1.0
stoichiometry:c9 : 1
stoichiometry:c10 : 1
stoichiometry:c11 : 1

m27*m11*0.1

nodelay

true

false

PMID: 1373512, 1698311 Complexes of LPS and LBP now seem to be recognized and specifically bound by a cellular receptor on myeloid cells, the surface antigen CD14.

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639.0, 771.0

25.0, 25.0

cso30:i:ME_Translation

cso30:i:CC_Cytosol

e25 e28 e30 e29

e26

and

mass

coefficient1:0.1
coefficient2:1.0
stoichiometry:c12 : 1
stoichiometry:c14 : 1
stoichiometry:c40 : 1
stoichiometry:c18 : 1
stoichiometry:c13 : 1

m25*m28*0.1

nodelay

true

false

PMID: 1373512, 1698311 In experiments that gave first evidence for CD14 being the receptor for LBP/LPS complexes, anti-CD 14 mAb 3C IO and 6Ob were able to inhibit LBP/LPS-mediated effects, including TNF production and opsonin function.

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1251.0, 91.0

25.0, 25.0

cso30:i:ME_Binding

cso30:i:CC_Extracellular

e5 e31

e32

and

mass

coefficient1:0.1
coefficient2:1.0
stoichiometry:c21 : 1
stoichiometry:c22 : 1
stoichiometry:c23 : 1

m5*m31*0.1

nodelay